Beilstein J. Org. Chem.2017,13, 1498–1506, doi:10.3762/bjoc.13.148
over micelle-forming detergents is demonstrated in the polymerization of phenylacetylene, showing a ten-fold increase in yield and increased molecular weights.
Keywords: amphiphilic molecule 2-methyl-2,4-pentanediol; hybrid catalyst; phenylacetylenepolymerization; refolding agents; transmembrane
demonstrate the importance of the right choice of the membrane protein stabilizer for biohybrid catalysis.
Results and Discussion
For solubilizing the transmembrane protein FhuA ΔCVFtev PE–PEG and MPD were applied as stabilizing agent and phenylacetylenepolymerization was performed as model reaction (Figure
the biohybrid catalyst 2 in phenylacetylenepolymerization. The small detergent MPD stabilizes the transmembrane protein FhuA ΔCVFtev in aqueous solution without forming micelles. The structural integrity was proven by CD spectroscopy. Applying MPD as stabilizing agent, an approximately ten-fold
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Graphical Abstract
Figure 1:
Phenylacetylene polymerization of FhuA ΔCVFtev [29] refolded in a polymer or small amphiphilic molecule...